![]() ![]() (F) 3D representation of (E) with stomatal cavity indicated (arrow). ROS production by ILL6002 in response to (C) AlKewell and (D) AlKewell82-GFP at one DPI (E) Surface growth of AlAlKewell82-GFP hyphae and stomatal entry (s). (A) Unlobed hyphae (h) at one DPI (B) Propidium iodide-stained ILL6002 infected with AlKewell82-GFP. ![]() Penetration structures and early colonization events. Scale bars are 50 μm, inset: scale bar is 10 μm. (E) Pathogenicity of AlKewell and AlKewell82-GFP on ILL6002 and ILL7537.įungal development during early stages of infection (A) Germination of conidia on the leaf cuticle at one 1 DPI (B) trichomes at 1 DPI (C) Formation of an appressorium (ap) at one DPI (D) AlKewell82-GFP mycelia after five DPI. Sporulation of (C) AlKewell and (D) AlKewell82-GFP on ILL6002. Radial growth and sporulation of (A) untransformed AlKewell and (B) AlKewell82-GFP. (A) Schematic diagram of tandem T-DNA sequences at the insertion site in AlKewell82-GFP prepared by SnapGene Viewer (GSL Biotech available at ) (B) PCR detection of the T-DNA and flanking host sequences using primers JD425 and JD381 (917 bp) and JD321 and JD427 (707 bp) for bands 1 and 2, respectively, and indicated in Fig 1A.Ĭharacterization of AlKewell82-GFP transformant. ![]()
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